ALS; ALS, amyotrophic lateral sclerosis; ANOVA, analysis of variance; Arg-1, arginine-1; Astrocyte; CM, conditioned medium; CNS, central nervous system; Cytokine; DMEM, Dulbecco's minimum essential medium; EAE, experimental autoimmune encephalomyelitis; GFAP, glial fibrillary acidic protein; HO-1, hemeoxygenase-1; IFN-γ, interferon gamma; IL-10, interleukin-10; IL-1RAcP, interleukin-1 receptor accessory protein; IL-33, interleukin-33; IL-33R, interleukin-33 receptor; IL-6, interleukin-6; Iba-1, ionized calcium binding adaptor molecule-1; Inflammation; Interleukin-33; MCP-1, monocyte chemoattractant protein-1; Microglia; NFE2L2, the gene encoding Nrf2; Nrf2, nuclear factor (erythroid-derived 2)-like 2; PBS, phosphate buffered saline; RT, room temperature; SC, spinal cord; SD, standard deviation; SOD1, superoxide dismutase 1; Spinal cord; T cell; TG, transgenic; TNF, tumor necrosis factor; WT, wildtype; fALS, familial ALS; sALS, sporadic ALS