S100A8/A9 in Myocardial Infarction.
Methods Mol Biol
; 1929: 739-754, 2019.
Article
em En
| MEDLINE
| ID: mdl-30710308
ABSTRACT
S100A8/A9 represents a novel biomarker and therapeutic target in sterile inflammatory diseases. Among the various S100 proteins, S100A8 and S100A9 have been shown to be the most important of all the damage-associated molecular pattern (DAMP) proteins in sterile inflammatory conditions such as diabetes, cardiovascular disease, autoimmune disorders, etc. We present here methods to quantify S100A8/A9 expression in various tissues in mouse models of myocardial infarction (MI) using flow cytometry (FC), immunofluorescence, quantitative real-time polymerase chain reaction (q-RT-PCR), and enzyme-linked immunosorbent assays (ELISA).
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Calgranulina A
/
Calgranulina B
/
Infarto do Miocárdio
Limite:
Animals
/
Humans
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article