3D-Embedded Cell Cultures to Study Tendon Biology.
Methods Mol Biol
; 2045: 155-165, 2019.
Article
em En
| MEDLINE
| ID: mdl-30734212
ABSTRACT
Tendons harbor various cell populations, including cells displaying classical adult mesenchymal stromal cell criteria. Previous studies have shown that a tenogenic phenotype is more effectively maintained in a 3D cell culture model under mechanical load. This chapter describes a method to isolate tendon-derived cells from rat Achilles tendons and the subsequent formation of 3D-embedded cell cultures. These tendon-like constructs can then be analyzed by various means, including histology, immunohistochemistry, qPCR, or standard protein analysis techniques.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Tendão do Calcâneo
/
Células-Tronco
/
Organoides
/
Diferenciação Celular
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Técnicas de Cultura de Células
/
Engenharia Tecidual
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article