Ultrasound-enhanced scintillation proximity assay for rapid diagnostics.
Anal Biochem
; 570: 51-55, 2019 04 01.
Article
em En
| MEDLINE
| ID: mdl-30771337
ABSTRACT
Scintillation proximity assay (SPA) is a type of radioimmunoassay (RIA). We apply ultrasound enhancement to the general SPA. All assay procedures, including the antibody coating and radiolabeled antigen binding are achieved by simply mixing then standing for 5â¯min in an ultrasound chamber. No additional incubation time is required. To further demonstrate the capability of the UE-SPA, a quantitative measurement of CD55 in various grades of colon tumors was assessed on human tissue slides. The results showed a significant correlation between CD55 expression and tumorigenesis. In conclusion, we confirmed that UE-SPA is a reliable, rapid and alternative to RIA.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Radioimunoensaio
/
Antígenos CD55
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article