Microparticles of Lamivudine-Poly-ε-Caprolactone Conjugate for Drug Delivery via Internalization by Macrophages.
Molecules
; 24(4)2019 Feb 17.
Article
em En
| MEDLINE
| ID: mdl-30781578
ABSTRACT
The past decade may be considered as revolutionary in the research field focused on the physiological function of macrophages. Unknown subtypes of these cells involved in pathological mechanisms were described recently, and they are considered as potential drug delivery targets. The innate ability to internalize foreign bodies exhibited by macrophages can be employed as a therapeutic strategy. The efficiency of this uptake depends on the size, shape and surface physiochemical properties of the phagocyted objects. Here, we propose a method of preparation and preliminary evaluation of drug-polymer conjugate-based microspheres for macrophage targeted drug delivery. The aim of the study was to identify crucial uptake-enhancing parameters for solid, surface modified particles. A model drug molecule-lamivudine-was conjugated with poly-ε-caprolactone via ring opening polymerization. The conjugate was utilized in a solvent evaporation method technique to form solid particles. Interactions between particles and a model rat alveolar cell line were evaluated by flow cytometry. The polymerization product was characterized by a molecular weight of 3.8 kDa. The surface of the obtained solid drug-loaded cores of a hydrodynamic diameter equal to 2.4 µm was modified with biocompatible polyelectrolytes via a layer-by-layer assembly method. Differences in the internalization efficiency of four particle batches by the model RAW 264.7 cell line suggest that particle diameter and surface hydrophobicity are the most influential parameters in terms of phagocytic uptake.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Caproatos
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Portadores de Fármacos
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Sistemas de Liberação de Medicamentos
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Lamivudina
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Fármacos Anti-HIV
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Lactonas
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Macrófagos
Limite:
Animals
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article