Agar pre-embedding of small skin biopsies: real-life benefits and challenges in high throughput pathology laboratories.
J Clin Pathol
; 72(6): 448-451, 2019 Jun.
Article
em En
| MEDLINE
| ID: mdl-30787027
ABSTRACT
Paraffin embedding of small, thin tissue samples requires specific expertise for optimal orientation before tissue sectioning. This study evaluates the real-life utility of the agar pre-embedding technique for small skin biopsies with regards to lengthening of work times, problems in orientation (re-embedding) and ancillary techniques (immunohistochemistry and in situ hybridisation) between two high work flow pathology laboratories, one of which routinely uses the agar pre-embedding technique and one which does not. The mean time required for pre-embedding in agar was 30.4 s, but time for paraffin embedding for agar pre-embedded samples was shorter than the traditional method (177 vs 296 s; p<0.005). The number of skin samples requiring re-embedding was significantly higher with the traditional embedding method (p<0.005). No problems in immunoreactivity were observed in all 1900 reactions performed with 17 different antibodies. Fluorescence in situ hybridisation analysis was optimised with a prolonged protease K incubation time (21 vs 18 min).
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Patologia Clínica
/
Pele
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Neoplasias Cutâneas
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Imuno-Histoquímica
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Biomarcadores Tumorais
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Inclusão em Parafina
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Hibridização in Situ Fluorescente
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Ágar
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Ensaios de Triagem em Larga Escala
Tipo de estudo:
Evaluation_studies
/
Prognostic_studies
Limite:
Humans
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article