Application of a multiphase microreactor chemostat for the determination of reaction kinetics of Staphylococcus carnosus.
Bioprocess Biosyst Eng
; 42(6): 953-961, 2019 Jun.
Article
em En
| MEDLINE
| ID: mdl-30810809
ABSTRACT
Bioreactors at the microliter scale offer a promising approach to accelerate bioprocess development. Advantages of such microbioreactors include a reduction in the use of expensive reagents. In this study, a chemostat operation mode of a cuvette-based microbubble column bioreactor made of polystyrene (working volume of 550 µL) was demonstrated. Aeration occurs through a nozzle (Ø ≤ 100 µm) and supports submerged whole-cell cultivation of Staphylococcus carnosus. Stationary concentrations of biomass and glucose were determined in the dilution rate regime ranging from 0.12 to 0.80 1/h with a glucose feed concentration of 1 g/L. For the first time, reaction kinetics of S. carnosus were estimated from data obtained from continuous cultivation. The maximal specific growth rate (µmax = 0.824 1/h), Monod constant (KS = 34 × 10- 3gS/L), substrate-related biomass yield coefficient (YX/S = 0.315 gCDW/gS), and maintenance coefficient (mS = 0.0035 gS/(gCDW·h)) were determined. These parameters are now available for further studies in the field of synthetic biology.
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MEDLINE
Assunto principal:
Staphylococcus
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Biomassa
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Reatores Biológicos
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article