The adequate amount of sodium chloride in Protein A wash buffer for effective host cell protein clearance.
Protein Expr Purif
; 158: 59-64, 2019 06.
Article
em En
| MEDLINE
| ID: mdl-30825515
ABSTRACT
Post-load column wash in Protein A chromatography can effectively improve host cell protein (HCP) clearance. A commonly used wash additive for this purpose is sodium chloride. However, the adequate amount of sodium chloride required for effective HCP clearance is less consistent in literature. In this study we investigated the impact of different amounts of sodium chloride on HCP clearance with five monoclonal antibodies (mAbs). For each mAb, elution pool HCP levels from runs under different wash conditions are compared. For all five mAbs, the data suggested that 250â¯mM would be an adequate amount for the salt to largely achieve its HCP reducing effect. The same conclusion is also reached for calcium chloride, a less commonly used but equally effective Protein A wash additive for HCP clearance.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Proteína Estafilocócica A
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Cloreto de Sódio
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Cromatografia de Afinidade
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Anticorpos Monoclonais
Limite:
Animals
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article