Chemiluminescence of canine peripheral blood lymphocytes stimulated by mitogens.
Vet Immunol Immunopathol
; 11(2): 175-92, 1986 Feb.
Article
em En
| MEDLINE
| ID: mdl-3083576
ABSTRACT
Luminol-dependent chemiluminescence of peripheral blood lymphocytes from dogs stimulated with concanavalin A (Con A) or phytohemagglutinin P (PHA) was measured with a Pico-Lite luminometer. 10 microliter of luminol gave optimal quantum yield from 1 X 10(6) lymphocytes sensitized with either 80 micrograms Con A or 160 micrograms PHA. Addition of superoxide dismutase did not influence the course of chemiluminescence. Whereas catalase produced 41% increase in quantum yield, mannitol caused a 51% inhibition of chemiluminescence. Lymphocytes exposed to varying doses of short term x-irradiation or lymphocytes isolated from dogs kept under continuous exposure through a gamma irradiation source showed dose-related depression of chemiluminescence. Membrane factors may be involved in lymphocyte stimulation to chemiluminescence as pulse experiments with Con A and PHA revealed. It is proposed that chemiluminescence measurements may be useful in monitoring early events in lymphocyte stimulation by antigens and mitogens.
Buscar no Google
Base de dados:
MEDLINE
Assunto principal:
Piridazinas
/
Ativação Linfocitária
/
Medições Luminescentes
/
Luminol
Limite:
Animals
Idioma:
En
Ano de publicação:
1986
Tipo de documento:
Article