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Solid-phase synthesis of molecularly imprinted polymer nanolabels: Affinity tools for cellular bioimaging of glycans.
Medina Rangel, Paulina X; Laclef, Sylvain; Xu, Jingjing; Panagiotopoulou, Maria; Kovensky, José; Tse Sum Bui, Bernadette; Haupt, Karsten.
Afiliação
  • Medina Rangel PX; Sorbonne Universités, Université de Technologie de Compiègne, CNRS Enzyme and Cell Engineering Laboratory, Rue Roger Couttolenc, CS 60319, 60203, Cedex, Compiègne, France.
  • Laclef S; Université de Picardie Jules Verne, Laboratoire de Glycochimie, des Antimicrobiens et des Agroressources, UMR CNRS 7378, 33 rue Saint Leu, 80039, cedex, Amiens, France.
  • Xu J; Sorbonne Universités, Université de Technologie de Compiègne, CNRS Enzyme and Cell Engineering Laboratory, Rue Roger Couttolenc, CS 60319, 60203, Cedex, Compiègne, France.
  • Panagiotopoulou M; Sorbonne Universités, Université de Technologie de Compiègne, CNRS Enzyme and Cell Engineering Laboratory, Rue Roger Couttolenc, CS 60319, 60203, Cedex, Compiègne, France.
  • Kovensky J; Université de Picardie Jules Verne, Laboratoire de Glycochimie, des Antimicrobiens et des Agroressources, UMR CNRS 7378, 33 rue Saint Leu, 80039, cedex, Amiens, France. jose.kovensky@u-picardie.fr.
  • Tse Sum Bui B; Sorbonne Universités, Université de Technologie de Compiègne, CNRS Enzyme and Cell Engineering Laboratory, Rue Roger Couttolenc, CS 60319, 60203, Cedex, Compiègne, France. jeanne.tse-sum-bui@utc.fr.
  • Haupt K; Sorbonne Universités, Université de Technologie de Compiègne, CNRS Enzyme and Cell Engineering Laboratory, Rue Roger Couttolenc, CS 60319, 60203, Cedex, Compiègne, France. karsten.haupt@utc.fr.
Sci Rep ; 9(1): 3923, 2019 03 08.
Article em En | MEDLINE | ID: mdl-30850730
ABSTRACT
Hyaluronic acid (HA) is a glycosaminoglycan that plays many roles in health and disease and is a key biomarker of certain cancers. Therefore, its detection at an early stage, by histochemical methods, is of importance. However, intracellular HA can be masked by other HA-binding macromolecules, rendering its visualization somehow problematic. We show that fluorescent molecularly imprinted polymer nanogels (MIP-NPs), can localize and detect intracellular HA. MIP-NPs were synthesized by solid-phase synthesis on glass beads (GBs). GBs were functionalized with terminal alkyne groups on which an azide derivative of the template molecule glucuronic acid was immobilized via click chemistry. Immobilization via the anomeric carbon left the template's carboxyl moiety free to enable strong stoichiometric electrostatic interactions with a benzamidine-based functional monomer, to confer selective recognition to the MIP-NPs. Due to the two-point orientation of the template, the resulting MIP-NPs were endowed with improved binding site homogeneity and specificity, reminiscent of monoclonal antibodies. These synthetic antibodies were then applied for probing and staining HA, of which glucuronic acid is a substructure (epitope), on human epidermal cells. Their excellent sensitivity, small size and water compatibility, enabled the MIP-NPs to visualize HA, as evidenced by confocal fluorescence micrographs.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Polímeros / Polissacarídeos / Impressão Molecular / Técnicas de Síntese em Fase Sólida / Nanogéis Limite: Humans Idioma: En Ano de publicação: 2019 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Polímeros / Polissacarídeos / Impressão Molecular / Técnicas de Síntese em Fase Sólida / Nanogéis Limite: Humans Idioma: En Ano de publicação: 2019 Tipo de documento: Article