Does Ralstonia eutropha, rich in poly-ß hydroxybutyrate (PHB), protect blue mussel larvae against pathogenic vibrios?
J Fish Dis
; 42(6): 777-787, 2019 Jun.
Article
em En
| MEDLINE
| ID: mdl-30850999
ABSTRACT
The natural amorphous polymer poly-ß-hydroxybutyrate (PHB-A lyophilized Ralstonia eutropha containing 75% PHB) was used as a biological agent to control bacterial pathogens of blue mussel (Mytilus edulis) larvae. The larvae were supplied with PHB-A at a concentration of 1 or 10 mg/L for 6 or 24 hr, followed by exposure to either the rifampicin-resistant pathogen Vibrio splendidus or Vibrio coralliilyticus at a concentration of 105 CFU/ml. Larvae pretreated 6 hr with PHB-A (1 mg/L) survived a Vibrio challenge better relative to 24 hr pretreatment. After 96 hr of pathogen exposure, the survival of PHB-A-treated mussel larvae was 1.41- and 1.76-fold higher than the non-treated larvae when challenged with V. splendidus and V. coralliilyticus, respectively. Growth inhibition of the two pathogens at four concentrations of the monomer ß-HB (1, 5, 25 and 125 mM) was tested in vitro in LB35 medium, buffered at two different pH values (pH 7 and pH 8). The highest concentration of 125 mM significantly inhibited the pathogen growth in comparison to the lower levels. The effect of ß-HB on the production of virulence factors in the tested pathogenic Vibrios revealed a variable pattern of responses.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Poliésteres
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Vibrio
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Vibrioses
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Cupriavidus necator
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Mytilus edulis
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Hidroxibutiratos
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Antibacterianos
Limite:
Animals
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article