Nanoscale exploration of the extracellular space in the live brain by combining single carbon nanotube tracking and super-resolution imaging analysis.
Methods
; 174: 91-99, 2020 03 01.
Article
em En
| MEDLINE
| ID: mdl-30862507
ABSTRACT
The brain extracellular space (ECS) is a system of narrow compartments whose intricate nanometric structure has remained elusive until very recently. Understanding such a complex organisation represents a technological challenge that requires a technique able to resolve these nanoscopic spaces and simultaneously characterize their rheological properties. We recently used single-walled carbon nanotubes (SWCNTs) as near-infrared fluorescent probes to map with nanoscale precision the local organization and rheology of the ECS. Here we expand our method by tracking single nanotubes through super-resolution imaging in rat organotypic hippocampal slices and acute brain slices from adult mice, pioneering the exploration of the adult brain ECS at the nanoscale. We found a highly heterogeneous ECS, where local rheological properties can change drastically within few nanometres. Our results suggest differences in local ECS diffusion environments in organotypic slices when compared to adult mouse slices. Data obtained from super-resolved maps of the SWCNT trajectories indicate that ECS widths may vary between brain tissue models, with a looser, less crowded nano-environment in organotypic cultured slices.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Encéfalo
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Nanotubos de Carbono
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Espaço Extracelular
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Microscopia Intravital
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Imagem Individual de Molécula
Limite:
Animals
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article