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Surface Modifications for Photon-Upconversion-Based Energy-Transfer Nanoprobes.
Andresen, Elina; Resch-Genger, Ute; Schäferling, Michael.
Afiliação
  • Andresen E; BAM Federal Institute of Materials Research and Testing, Biophotonics Division, Richard-Willstätter-Str. 11 , D-12489 Berlin , Germany.
  • Resch-Genger U; Department of Chemistry , Humboldt-Universität zu Berlin , Brook-Taylor-Str. 2 , D-12489 Berlin , Germany.
  • Schäferling M; BAM Federal Institute of Materials Research and Testing, Biophotonics Division, Richard-Willstätter-Str. 11 , D-12489 Berlin , Germany.
Langmuir ; 35(15): 5093-5113, 2019 04 16.
Article em En | MEDLINE | ID: mdl-30870593
An emerging class of inorganic optical reporters are near-infrared (NIR) excitable lanthanide-based upconversion nanoparticles (UCNPs) with multicolor emission and long luminescence lifetimes in the range of several hundred microseconds. For the design of chemical sensors and optical probes that reveal analyte-specific changes in their spectroscopic properties, these nanomaterials must be combined with sensitive indicator dyes that change their absorption and/or fluorescence properties selectively upon interaction with their target analyte, utilizing either resonance energy transfer (RET) processes or reabsorption-related inner filter effects. The rational development of UCNP-based nanoprobes for chemical sensing and imaging in a biological environment requires reliable methods for the surface functionalization of UCNPs, the analysis and quantification of surface groups, a high colloidal stability of UCNPs in aqueous media as well as the chemically stable attachment of the indicator molecules, and suitable instrumentation for the spectroscopic characterization of the energy-transfer systems and the derived nanosensors. These topics are highlighted in the following feature article, and examples of functionalized core-shell nanoprobes for the sensing of different biologically relevant analytes in aqueous environments will be presented. Special emphasis is placed on the intracellular sensing of pH.

Texto completo: 1 Base de dados: MEDLINE Idioma: En Ano de publicação: 2019 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Idioma: En Ano de publicação: 2019 Tipo de documento: Article