Silibinin's regulation of proliferation and collagen gene expressions of rat pancreatic ß-cells cultured on types I and V collagen involves ß-catenin nuclear translocation.
Connect Tissue Res
; 60(5): 463-476, 2019 09.
Article
em En
| MEDLINE
| ID: mdl-30871385
ABSTRACT
Extracellular matrix (ECM) molecules have multiple functions; prevention of cytotoxicity, provision of mechanical support, cell adhesive substrates and structural integrity in addition to mediation of cellular signaling. In this study, we report that the proliferation of INS-1 cells cultured on collagen I-coated dishes is enhanced, but it is inhibited on collagen V-coated dishes. Inhibitory proliferation on collagen V-coated is not due to apoptosis induction. Silibinin decreases hepatic glucose production and protects pancreatic ß-cells, as a potential medicine for type II diabetes. Silibinin up-regulates the proliferation of cells cultured on both collagen I- and V-coated dishes. Collagen-coating regulates gene expression of collagen in a collagen type-related manner. Silibinin increases mRNA expression of collagen I in the cells on collagen I- and V-coated dishes; however, silibinin decreases collagen V mRNA expression on collagen I- and V-coated dishes. Collagen I-coating significantly enhances nuclear translocation of ß-catenin, while collagen V-coating reduces it. Differential effects of silibinin on collagen I mRNA and collagen V mRNA can be accounted for by the finding that silibinin enhances nuclear translocation of ß-catenin on both collagen I- and V-coated dishes, since phenomenologically nuclear translocation of ß-catenin enhances collagen I mRNA but represses collagen V mRNA. These results demonstrate that nuclear translocation of ß-catenin up-regulates proliferation and collagen I gene expression, whereas it down-regulates collagen V gene expression of INS-1 cells. Differential gene expressions of collagen I and V by nuclear ß-catenin could be important for understanding fibrosis where collagen I and V may have differential effects.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Núcleo Celular
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Regulação da Expressão Gênica
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Colágeno Tipo I
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Colágeno Tipo V
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Células Secretoras de Insulina
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Beta Catenina
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Silibina
Tipo de estudo:
Qualitative_research
Limite:
Animals
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article