A SERS nano-tag-based magnetic-separation strategy for highly sensitive immunoassay in unprocessed whole blood.
Talanta
; 198: 527-533, 2019 Jun 01.
Article
em En
| MEDLINE
| ID: mdl-30876595
ABSTRACT
Assay technologies capable of detecting biomarker concentrations in unprocessed whole blood samples are fundamental for applications in medical diagnostics. SERS nano-tags integrated magnetic-separation biosensor (MSB) was realized for the first time for immunoassay in whole blood. The reliability and sensitivity of this method rely, in a large extent, on the quality and properties of the SERS nano-tags. The constructed silicacoated Ag SERS nano-tags as labels were used in a rapid and specific MSB immune sensor to detect Matrix Metalloproteinases 9 (MMP-9) in unprocessed blood samples. With fast screening ability and outstanding sensitivity, we anticipate that this method would greatly promote practical application of stroke-based early-stage cancer diagnosis.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Imunoensaio
/
Metaloproteinase 9 da Matriz
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article