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Effects of formaldehyde on detoxification and immune responses in silver pomfret (Pampus argenteus).
Hu, Jiabao; Le, Qijun; Wang, Yajun; Yu, Na; Cao, Xiaohuan; Kuang, Siwen; Zhang, Man; Gu, Weiwei; Sun, Yibo; Yang, Yang; Xu, Shanliang; Yan, Xiaojun.
Afiliação
  • Hu J; Key Laboratory of Applied Marine Biotechnology, Ningbo University, Ministry of Education, Ningbo, China; Key Laboratory of Marine Biotechnology of Zhejiang Province, Ningbo University, Ningbo, China; College of Marine Sciences, Ningbo University, Ningbo, China.
  • Le Q; Key Laboratory of Applied Marine Biotechnology, Ningbo University, Ministry of Education, Ningbo, China; Key Laboratory of Marine Biotechnology of Zhejiang Province, Ningbo University, Ningbo, China; College of Marine Sciences, Ningbo University, Ningbo, China; Ningbo Entry-Exit Inspection and Quara
  • Wang Y; Key Laboratory of Applied Marine Biotechnology, Ningbo University, Ministry of Education, Ningbo, China; Key Laboratory of Marine Biotechnology of Zhejiang Province, Ningbo University, Ningbo, China; College of Marine Sciences, Ningbo University, Ningbo, China. Electronic address: wangyajun@nbu.edu.
  • Yu N; Key Laboratory of Applied Marine Biotechnology, Ningbo University, Ministry of Education, Ningbo, China; Key Laboratory of Marine Biotechnology of Zhejiang Province, Ningbo University, Ningbo, China; College of Marine Sciences, Ningbo University, Ningbo, China.
  • Cao X; Key Laboratory of Applied Marine Biotechnology, Ningbo University, Ministry of Education, Ningbo, China; Key Laboratory of Marine Biotechnology of Zhejiang Province, Ningbo University, Ningbo, China; College of Marine Sciences, Ningbo University, Ningbo, China.
  • Kuang S; Key Laboratory of Applied Marine Biotechnology, Ningbo University, Ministry of Education, Ningbo, China; Key Laboratory of Marine Biotechnology of Zhejiang Province, Ningbo University, Ningbo, China; College of Marine Sciences, Ningbo University, Ningbo, China.
  • Zhang M; Key Laboratory of Applied Marine Biotechnology, Ningbo University, Ministry of Education, Ningbo, China; Key Laboratory of Marine Biotechnology of Zhejiang Province, Ningbo University, Ningbo, China; College of Marine Sciences, Ningbo University, Ningbo, China.
  • Gu W; Key Laboratory of Applied Marine Biotechnology, Ningbo University, Ministry of Education, Ningbo, China; Key Laboratory of Marine Biotechnology of Zhejiang Province, Ningbo University, Ningbo, China; College of Marine Sciences, Ningbo University, Ningbo, China.
  • Sun Y; Key Laboratory of Applied Marine Biotechnology, Ningbo University, Ministry of Education, Ningbo, China; Key Laboratory of Marine Biotechnology of Zhejiang Province, Ningbo University, Ningbo, China; College of Marine Sciences, Ningbo University, Ningbo, China.
  • Yang Y; Key Laboratory of Applied Marine Biotechnology, Ningbo University, Ministry of Education, Ningbo, China; Key Laboratory of Marine Biotechnology of Zhejiang Province, Ningbo University, Ningbo, China; College of Marine Sciences, Ningbo University, Ningbo, China.
  • Xu S; Key Laboratory of Applied Marine Biotechnology, Ningbo University, Ministry of Education, Ningbo, China; Key Laboratory of Marine Biotechnology of Zhejiang Province, Ningbo University, Ningbo, China; College of Marine Sciences, Ningbo University, Ningbo, China.
  • Yan X; Key Laboratory of Applied Marine Biotechnology, Ningbo University, Ministry of Education, Ningbo, China; Key Laboratory of Marine Biotechnology of Zhejiang Province, Ningbo University, Ningbo, China; College of Marine Sciences, Ningbo University, Ningbo, China. Electronic address: yanxiaojun@nbu.edu
Fish Shellfish Immunol ; 88: 449-457, 2019 May.
Article em En | MEDLINE | ID: mdl-30877061
ABSTRACT
Formaldehyde can effectively control ectoparasites in silver pomfret (Pampus argenteus). However, there is limited information on the effects of formaldehyde treatment at a molecular level in fishes. In the present study, transcriptome profiling was conducted to investigate the effects of formaldehyde treatment (80 mg/L, bath for 1 h every day for three consecutive days) on the liver and kidney tissues of silver pomfret. A total of 617959982 clean reads were obtained and assembled into 265760 unigenes with an N50 length of 1507 bp, and the assembled unigenes were all annotated by alignment with public databases. A total of 2204 differentially expressed genes (DEGs) were detected in the liver and kidney tissues, and they included 7 detoxification- related genes and 9 immune-related genes, such as CYP450, GST, MHC I & II, and CCR. In addition, 1440 DEGs were mapped to terms in the GO database, and 1064 DEGs were mapped to the KEGG database. The expression of 4 detoxification-related genes and 6 immune-related genes in three days formaldehyde treatment were analyzed using RT-qPCR, and the antioxidant enzyme levels were also determined. The results indicate differential expression of detoxification- and immune-related genes during the three days formaldehyde treatment. Our data could provide a reference for the treatment of parasites to avoid high mortality and help in understanding the molecular activity in fishes after formaldehyde exposure.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Perciformes / Inativação Metabólica / Transcriptoma / Formaldeído Limite: Animals Idioma: En Ano de publicação: 2019 Tipo de documento: Article
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Perciformes / Inativação Metabólica / Transcriptoma / Formaldeído Limite: Animals Idioma: En Ano de publicação: 2019 Tipo de documento: Article