NanoBioAnalytical characterization of extracellular vesicles in 75-nm nanofiltered human plasma for transfusion: A tool to improve transfusion safety.
Nanomedicine
; 20: 101977, 2019 08.
Article
em En
| MEDLINE
| ID: mdl-30878658
ABSTRACT
Plasma transfusion induces some transfusion related acute lung injury (TRALI) mediated through neutrophil extracellular traps (NETs). We investigated whether extracellular vesicles (EVs) present in plasma or obtained from resting (N-PEVs) or thrombin activated platelets (T-PEVs) can trigger NETs, and whether 75â¯nm-nanofiltration, to partially remove EVs, prohibits NETs formation. EVs size and concentration were determined by conventional biophysical approaches and by an original NanoBioAnalytical (NBA) platform based on EV immunocapture biochip, combining Surface Plasmon Resonance Imaging (SPRi) and Atomic Force Microscopy (AFM) exploration. EVs effective diameter was in the 25-1000â¯nm range, with a majority (≈ 90%)â¯≤â¯100â¯nm. Both T-PEVs in buffer (but not N-PEVs) and non-nanofiltered plasma containing T-PEVs triggered NETs formation. Nanofiltration depleted large EVs (> 70â¯nm) and decreased NETs formation. The NBA platform was found to be a suitable tool to investigate the safety of plasma for transfusion.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Transfusão de Sangue
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Nanotecnologia
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Vesículas Extracelulares
Limite:
Humans
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article