The effect of lithium chloride on BDNF, NT3, and their receptor mRNA levels in the spinal contusion rat models.
Neurol Res
; 41(6): 577-583, 2019 Jun.
Article
em En
| MEDLINE
| ID: mdl-30879425
ABSTRACT
OBJECTIVE:
Nowadays, there seems to be no decisive way for treatment of spinal cord injury (SCI).Extensive cell death (apoptosis and necrosis) occurring in SCI can cause considerable progressive sensorimotor disabilities. Preventing cell death by improving endogenous regenerative capability could an effective strategy for the treatment of SCI. This study was designed to evaluate the effects of lithium chloride (LiCl) on the cell survival through overexpression of BDNF and NT3 mRNA level and their receptors in the contusion rat models.METHODS:
Rats were randomly divided into four experimental groups (eight rats/group) including contused animals (the non-treatment group); contused animals (the control group) which received laminectomy; contused animals received normal saline (vehicle)and contused animals received intraperitoneal injection of 20 mg/kg LiCl three days after surgery. Injection continued for 14 days as treatment. Basso, Beattie, Bresnahan (BBB) rating scale was used to assess the motor function of the rats. To evaluate the histopathological and gene expression analysis, rats were sacrificed 28 days after surgery. Real-time reverse transcriptase polymerase chain reaction (RT-PCR) was performed to obtain the relative levels of mRNA for BDNF, NT3 and their receptors.RESULTS:
The results showed LiCl ameliorates BBB scores via up-regulation of BDNF and TrkB receptors. Also, histological analysis showed that the numerical density per area of TUNEL- positive cells and the percentage of cavity significantly decreased in the LiCl-treated group.CONCLUSION:
Our findings suggest that LiCl protects neural cells and effectively enhances locomotor function, which was done through up-regulation of endogenous BDNF expression in rats with SCI. ABBREVIATIONS SCI spinal cord injury; LiCl lithium chloride; BDNF Brain-derived neurotrophic factor; NT3 Neurotrophin-3; BBB Basso, Beattie, Bresnahan; TrkB Tropomyosin receptor kinase B; TUNEL Terminal deoxynucleotidyl transferase dUTP nick end labeling.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Traumatismos da Medula Espinal
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Cloreto de Lítio
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Fator Neurotrófico Derivado do Encéfalo
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Contusões
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article