Enhanced Cas12a editing in mammalian cells and zebrafish.

Liu, Pengpeng; Luk, Kevin; Shin, Masahiro; Idrizi, Feston; Kwok, Samantha; Roscoe, Benjamin; Mintzer, Esther; Suresh, Sneha; Morrison, Kyle; Frazão, Josias B; Bolukbasi, Mehmet Fatih; Ponnienselvan, Karthikeyan; Luban, Jeremy; Zhu, Lihua Julie; Lawson, Nathan D; Wolfe, Scot A

Liu, Pengpeng; Luk, Kevin; Shin, Masahiro; Idrizi, Feston; Kwok, Samantha; Roscoe, Benjamin; Mintzer, Esther; Suresh, Sneha; Morrison, Kyle; Frazão, Josias B; Bolukbasi, Mehmet Fatih; Ponnienselvan, Karthikeyan; Luban, Jeremy; Zhu, Lihua Julie; Lawson, Nathan D; Wolfe, Scot A.

Afiliação

Liu P; Department of Molecular, Cell and Cancer Biology, University of Massachusetts Medical School, Worcester, Massachusetts, USA.
Luk K; Department of Molecular, Cell and Cancer Biology, University of Massachusetts Medical School, Worcester, Massachusetts, USA.
Shin M; Department of Molecular, Cell and Cancer Biology, University of Massachusetts Medical School, Worcester, Massachusetts, USA.
Idrizi F; Department of Molecular, Cell and Cancer Biology, University of Massachusetts Medical School, Worcester, Massachusetts, USA.
Kwok S; Department of Molecular, Cell and Cancer Biology, University of Massachusetts Medical School, Worcester, Massachusetts, USA.
Roscoe B; Department of Molecular, Cell and Cancer Biology, University of Massachusetts Medical School, Worcester, Massachusetts, USA.
Mintzer E; Department of Molecular, Cell and Cancer Biology, University of Massachusetts Medical School, Worcester, Massachusetts, USA.
Suresh S; Department of Molecular, Cell and Cancer Biology, University of Massachusetts Medical School, Worcester, Massachusetts, USA.
Morrison K; Department of Chemistry and Biochemistry, Worcester Polytechnic Institute, Worcester, MA, USA.
Frazão JB; Department of Molecular, Cell and Cancer Biology, University of Massachusetts Medical School, Worcester, Massachusetts, USA.
Bolukbasi MF; Department of Molecular, Cell and Cancer Biology, University of Massachusetts Medical School, Worcester, Massachusetts, USA.
Ponnienselvan K; Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA, USA.
Luban J; Department of Molecular, Cell and Cancer Biology, University of Massachusetts Medical School, Worcester, Massachusetts, USA.
Zhu LJ; Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA, USA.
Lawson ND; Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA, USA.
Wolfe SA; Li Weibo Institute for Rare Diseases Research, University of Massachusetts Medical School, Worcester, MA, USA.

Nucleic Acids Res ; 47(8): 4169-4180, 2019 05 07.

Article em En | MEDLINE | ID: mdl-30892626

Assuntos

Sistemas CRISPR-Cas; Endonucleases/genética; Edição de Genes/métodos; RNA Guia de Cinetoplastídeos/genética; Ribonucleoproteínas/genética; Animais; Sequência de Bases; DNA (Citosina-5-)-Metiltransferase 1/genética; DNA (Citosina-5-)-Metiltransferase 1/metabolismo; Embrião não Mamífero; Endonucleases/metabolismo; Células HEK293; Células HeLa; Proteínas de Homeodomínio/genética; Proteínas de Homeodomínio/metabolismo; Humanos; Sequências Repetidas Invertidas; Células Jurkat; Células K562; Sinais de Localização Nuclear; Conformação de Ácido Nucleico; Plasmídeos/química; Plasmídeos/metabolismo; RNA Guia de Cinetoplastídeos/metabolismo; Ribonucleoproteínas/metabolismo; Fatores de Transcrição/genética; Fatores de Transcrição/metabolismo; Transfecção; Peixe-Zebra; Proteínas de Peixe-Zebra/genética; Proteínas de Peixe-Zebra/metabolismo

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Ribonucleoproteínas / RNA Guia de Cinetoplastídeos / Endonucleases / Sistemas CRISPR-Cas / Edição de Genes Limite: Animals / Humans Idioma: En Ano de publicação: 2019 Tipo de documento: Article

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