Expression, purification, and <i>in vitro</i> mitochondrial interaction analysis of full-length and truncated human tumor suppresser p53.

Wang, Lingyao; Zhao, Tong; Wang, Song; Jin, Jingji; Cai, Yong; Wang, Fei

Expression, purification, and in vitro mitochondrial interaction analysis of full-length and truncated human tumor suppresser p53.

Wang, Lingyao; Zhao, Tong; Wang, Song; Jin, Jingji; Cai, Yong; Wang, Fei.

Afiliação

Wang L; a School of Life Sciences , Jilin University , Changchun , Jilin , P.R. China.
Zhao T; a School of Life Sciences , Jilin University , Changchun , Jilin , P.R. China.
Wang S; a School of Life Sciences , Jilin University , Changchun , Jilin , P.R. China.
Jin J; a School of Life Sciences , Jilin University , Changchun , Jilin , P.R. China.
Cai Y; b National Engineering Laboratory for AIDS Vaccine , Jilin University , Changchun , Jilin , P.R. China.
Wang F; c Key Laboratory for Molecular Enzymology and Engineering, the Ministry of Education , Jilin University , Changchun , Jilin , P.R. China.

Biosci Biotechnol Biochem ; 83(7): 1220-1226, 2019 Jul.

Article em En | MEDLINE | ID: mdl-30898040

Assuntos

Mitocôndrias/metabolismo; Proteína Supressora de Tumor p53/isolamento & purificação; Proteína Supressora de Tumor p53/metabolismo; Apoptose/fisiologia; Eletroforese em Gel de Poliacrilamida; Escherichia coli/metabolismo; Humanos; Técnicas In Vitro; Proteínas Recombinantes/isolamento & purificação; Proteínas Recombinantes/metabolismo; Proteína Supressora de Tumor p53/fisiologia

Palavras-chave

His-tag fusion protein; P53; cell-free apoptosis analysis; soluble expression; truncation

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Proteína Supressora de Tumor p53 / Mitocôndrias Limite: Humans Idioma: En Ano de publicação: 2019 Tipo de documento: Article

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