Your browser doesn't support javascript.
loading
Gene expression profiles at different stages for formation of pearl sac and pearl in the pearl oyster Pinctada fucata.
Take, Saori; Igarashi, Yoji; Yoshitake, Kazutoshi; Asakawa, Shuichi; Maeyama, Kaoru; Nagai, Kiyohito; Watabe, Shugo; Kinoshita, Shigeharu.
Afiliação
  • Mariom; Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo, Tokyo, 113-8657, Japan.
  • Take S; Department of Fisheries Biology and Genetics, Faculty of Fisheries, Bangladesh Agricultural University, Mymensingh, 2202, Bangladesh.
  • Igarashi Y; Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo, Tokyo, 113-8657, Japan.
  • Yoshitake K; Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo, Tokyo, 113-8657, Japan.
  • Asakawa S; Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo, Tokyo, 113-8657, Japan.
  • Maeyama K; Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo, Tokyo, 113-8657, Japan.
  • Nagai K; Mikimoto Pharmaceutical CO., LTD, Kurose 1425, Ise, Mie, 516-8581, Japan.
  • Watabe S; Pearl Research Laboratory, K. MIKIMOTO & CO., LTD, Osaki Hazako 923, Hamajima, Shima, Mie, 517-0403, Japan.
  • Kinoshita S; School of Marine Biosciences, Kitasato University, Minami, Sagamihara, Kanagawa, 252-0313, Japan.
BMC Genomics ; 20(1): 240, 2019 Mar 25.
Article em En | MEDLINE | ID: mdl-30909870
BACKGROUND: The most critical step in the pearl formation during aquaculture is issued to the proliferation and differentiation of outer epithelial cells of mantle graft into pearl sac. This pearl sac secretes various matrix proteins to produce pearls by a complex physiological process which has not been well-understood yet. Here, we aimed to unravel the genes involved in the development of pearl sac and pearl, and the sequential expression patterns of different shell matrix proteins secreted from the pearl sac during pearl formation by pearl oyster Pinctada fucata using high-throughput transcriptome profiling. RESULTS: Principal component analysis (PCA) showed clearly different gene expression profiles between earlier (before 1 week) and later stages (1 week to 3 months) of grafting. Immune-related genes were highly expressed between 0 h - 24 h (donor dependent) and 48 h - 1 w (host dependent), and in the course of wound healing process pearl sac was developed by two weeks of graft transplantation. Moreover, for the first time, we identified some stem cell marker genes including ABCG2, SOX2, MEF2A, HES1, MET, NRP1, ESR1, STAT6, PAX2, FZD1 and PROM1 that were expressed differentially during the formation of pearl sac. The expression profiling of 192 biomineralization-related genes demonstrated that most of the shell matrix proteins (SMPs) involved in prismatic layer formation were first up-regulated and then gradually down-regulated indicating their involvement in the development of pearl sac and the onset of pearl mineralization. Most of the nacreous layer forming SMPs were up-regulated at 2 weeks after the maturation of pearl sac. Nacrein, MSI7 and shematrin involved in both layer formation were highly expressed during 0 h - 24 h, down-regulated up to 1 week and then up-regulated again after accomplishment of pearl sac formation. CONCLUSIONS: Using an RNA-seq approach we unraveled the expression pattern of the key genes involved in the development of pearl sac and pearl as a result of host immune response after grafting. These findings provide valuable information in understanding the molecular mechanism of pearl formation and immune response in P. fucata.
Assuntos
Palavras-chave

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Análise de Sequência de RNA / Perfilação da Expressão Gênica / Pinctada / Exoesqueleto Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Ano de publicação: 2019 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Análise de Sequência de RNA / Perfilação da Expressão Gênica / Pinctada / Exoesqueleto Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Ano de publicação: 2019 Tipo de documento: Article