Three-phase partitioning system with dimethyl carbonate as organic phase for partitioning of exopolysaccharides from Phellinus baumii.
Int J Biol Macromol
; 131: 941-948, 2019 Jun 15.
Article
em En
| MEDLINE
| ID: mdl-30914364
ABSTRACT
In this study, a three-phase partitioning (TPP) system with dimethyl carbonate (DMC) as organic phase and sodium citrate (SC) as salt phase was used for partitioning of exopolysaccharide (EPS), namely, EPS-D, from fermentation broth of Phellinus baumii. Results showed that the maximum extraction yield (EY) of EPS-D was 71.02% under the following modified optimal conditions DMC to fermentation broth ratio 0.51.0 (v/v), SC concentration 19% (w/v), temperature 30⯰C, and pHâ¯4.0. EPS-D had higher EY, carbohydrate, and uronic acid contents compared with the EPS, designated as EPS-T, obtained from the TPP system with t-butanol and ammonium sulfate. EPS-D and EPS-T had different chemical compositions and molecular weights; however, their preliminary chemical structures basically remained unchanged. Moreover, EPS-D exhibited stronger free radical-scavenging capability and total antioxidant capacity than EPS-T. Therefore, the TPP system with DMC as an alternative solvent for t-butanol has great potential for efficient partitioning of natural biomolecules.
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MEDLINE
Assunto principal:
Basidiomycota
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Extração Líquido-Líquido
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Polissacarídeos Fúngicos
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Formiatos
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article