Establishing cell painting in a smaller chemical biology lab - A report from the frontier.
Bioorg Med Chem
; 27(12): 2609-2615, 2019 06 15.
Article
em En
| MEDLINE
| ID: mdl-30935791
ABSTRACT
In this paper we will outline the efforts we have made recently to establish the profiling platform known as cell painting in our laboratory. This platform, which is based on fluorescence microscopy, allows rapid and cheap access to bioactivity fingerprints for small molecules and thereby can contribute with important information in many experimental situations that is faced in laboratories involved in molecular probe design, mode-of-action studies or that perform focused phenotypic screens. We have tried to achieve the following two objectives:
(1) provide a detailed description of the hurdles that we had to overcome during establishment and describe our final protocol; (2) provide a more pedagogical description of the different methods used to analyse and represent data from this experiment. Finally, we provide an example of how the method can be used to clarify mechanistic dichotomies.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Sondas Moleculares
/
Microscopia de Fluorescência
Limite:
Humans
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article