Structural basis of hypoxic gene regulation by the Rv0081 transcription factor of Mycobacterium tuberculosis.
FEBS Lett
; 593(9): 982-995, 2019 05.
Article
em En
| MEDLINE
| ID: mdl-30941756
ABSTRACT
The transcription factor Rv0081 of Mycobacterium tuberculosis controls hypoxic gene expression and acts as a regulatory hub in the latent phase of tuberculosis (TB) infection. We report here the crystal structure of Rv0081 at 2.9 Å resolution revealing that it belongs to the well-known ArsR/SmtB family proteins. However, unlike other members in this family, Rv0081 has neither a metal-binding domain nor does it possess Cys residues, suggesting an alternate mechanism of gene regulation. Our structural and biochemical analyses suggest the molecular basis for the recognition of self-regulatory DNA sequences and a plausible mechanism of regulation of Rv0081 in the latent phase of TB infection. DATABASE Structural data are available in the Protein Data Bank under the accession number - 6JMI.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Oxigênio
/
Proteínas de Bactérias
/
Fatores de Transcrição
/
Regulação Bacteriana da Expressão Gênica
/
Mycobacterium tuberculosis
Tipo de estudo:
Prognostic_studies
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article