The Gag protein PEG10 binds to RNA and regulates trophoblast stem cell lineage specification.
PLoS One
; 14(4): e0214110, 2019.
Article
em En
| MEDLINE
| ID: mdl-30951545
ABSTRACT
Peg10 (paternally expressed gene 10) is an imprinted gene that is essential for placental development. It is thought to derive from a Ty3-gyspy LTR (long terminal repeat) retrotransposon and retains Gag and Pol-like domains. Here we show that the Gag domain of PEG10 can promote vesicle budding similar to the HIV p24 Gag protein. Expressed in a subset of mouse endocrine organs in addition to the placenta, PEG10 was identified as a substrate of the deubiquitinating enzyme USP9X. Consistent with PEG10 having a critical role in placental development, PEG10-deficient trophoblast stem cells (TSCs) exhibited impaired differentiation into placental lineages. PEG10 expressed in wild-type, differentiating TSCs was bound to many cellular RNAs including Hbegf (Heparin-binding EGF-like growth factor), which is known to play an important role in placentation. Expression of Hbegf was reduced in PEG10-deficient TSCs suggesting that PEG10 might bind to and stabilize RNAs that are critical for normal placental development.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Placentação
/
Fatores de Transcrição
/
Proteínas Nucleares
/
Diferenciação Celular
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Fator de Crescimento Semelhante a EGF de Ligação à Heparina
Tipo de estudo:
Prognostic_studies
Limite:
Animals
/
Female
/
Humans
/
Pregnancy
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article