Effect of Liquid Nitrogen Flushing of Extender on Seminal Antioxidant Profile of Murrah Buffalo during Cryopreservation.
Cryo Letters
; 39(5): 279-287, 2018.
Article
em En
| MEDLINE
| ID: mdl-30963159
ABSTRACT
BACKGROUND:
The dissolved oxygen in the extender may act as a source for the production of reactive oxygen species that may lead to reduced seminal antioxidant profile which in turn may be responsible for impaired frozen thawed sperm quality and fertility.OBJECTIVE:
To study the effect of adding liquid nitrogen into the extender on semen freezability and seminal antioxidant profile in buffalo. MATERIALS ANDMETHODS:
Semen extender was prepared freshly and divided into two sub extenders namely, Extender I control (non deoxygenated) and Extender II partially deoxygenated by using LN2 flushing). The estimation of dissolved oxygen (DO) level was done in both extenders. Semen samples with mass motility of ≥ 3+ and individual progressive motility of 70% and above, collected from murrah buffalo bulls were utilized for the present study. Each semen sample was split into two group's viz., group I diluted with extender I and group II diluted with extender II up to 60×106 sperm/mL. The diluted semen samples were packed into French mini straws (0.25 mL), sealed with polyvinyl alcohol powder, kept for 3 h at 5°C for equilibration and then kept in automatic programmable freezer until temperature of straws reached -145°C followed by plunging into liquid nitrogen (-196°C). The evaluation of semen samples was carried out for various seminal attributes (sperm motility, live sperm count, acrosomal integrity, and hypo-osmotic swelling (HOS) response) and antioxidant profile (superoxide dismutase (SOD), glutathione peroxidase (GPx) and total antioxidant capacity (TAC)) at pre freeze and post thaw stage.RESULTS:
Sperm motility, live sperm count, acrosomal integrity, HOS response were significantly (P<0.05) higher in group II as compared to group I. The average seminal SOD, GPx and TAC levels were significantly (P<0.05) higher in group II as compared to group I at pre freeze and post thaw stage.CONCLUSION:
It is concluded that partial deoxygenation of the extender prior to its addition to semen enhances sperm quality in terms of sperm motility, live sperm count, acrosomal integrity, and hypo-osmotic swelling (HOS) response and also improves seminal antioxidant profile (superoxide dismutase (SOD), glutathione peroxidase (GPx) and total antioxidant capacity (TAC).
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Base de dados:
MEDLINE
Assunto principal:
Preservação do Sêmen
/
Espermatozoides
/
Criopreservação
/
Nitrogênio
/
Antioxidantes
Limite:
Animals
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article