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Predicted highly derived class 1 CRISPR-Cas system in Haloarchaea containing diverged Cas5 and Cas7 homologs but no CRISPR array.
Makarova, Kira S; Karamycheva, Svetlana; Shah, Shiraz A; Vestergaard, Gisle; Garrett, Roger A; Koonin, Eugene V.
Afiliação
  • Makarova KS; National Center for Biotechnology Information, National Library of Medicine, 8600 Rockville Pike, Bethesda, MD 20894, USA.
  • Karamycheva S; National Center for Biotechnology Information, National Library of Medicine, 8600 Rockville Pike, Bethesda, MD 20894, USA.
  • Shah SA; Danish Archaea Centre, Department of Biology, University of Copenhagen, Danish Archaea Centre, Ole Maaloes Vej 5, Copenhagen , DK-2200 Denmark.
  • Vestergaard G; Section for Bioinformatics, Department of Health Technology, Technical University of Denmark, Anker Engelunds Vej 1, 2800 Kgs. Lyngby, Denmark.
  • Garrett RA; Danish Archaea Centre, Department of Biology, University of Copenhagen, Danish Archaea Centre, Ole Maaloes Vej 5, Copenhagen , DK-2200 Denmark.
  • Koonin EV; National Center for Biotechnology Information, National Library of Medicine, 8600 Rockville Pike, Bethesda, MD 20894, USA.
FEMS Microbiol Lett ; 366(7)2019 04 01.
Article em En | MEDLINE | ID: mdl-30993331
Screening of genomic and metagenomic databases for new variants of CRISPR-Cas systems increasingly results in the discovery of derived variants that do not seem to possess the interference capacity and are implicated in functions distinct from adaptive immunity. We describe an extremely derived putative class 1 CRISPR-Cas system that is present in many Halobacteria and consists of distant homologs of the Cas5 and Cas7 protein along with an uncharacterized conserved protein and various nucleases. We hypothesize that, although this system lacks typical CRISPR effectors or a CRISPR array, it functions as a RNA-dependent defense mechanism that, unlike other derived CRISPR-Cas, utilizes alternative nucleases to cleave invader genomes.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Proteínas Arqueais / Genoma Arqueal / Halobacteriaceae Tipo de estudo: Prognostic_studies / Risk_factors_studies Idioma: En Ano de publicação: 2019 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Proteínas Arqueais / Genoma Arqueal / Halobacteriaceae Tipo de estudo: Prognostic_studies / Risk_factors_studies Idioma: En Ano de publicação: 2019 Tipo de documento: Article