Behavioral Tracking and Neuromast Imaging of Mexican Cavefish.
J Vis Exp
; (146)2019 04 06.
Article
em En
| MEDLINE
| ID: mdl-31009008
ABSTRACT
Cave-dwelling animals have evolved a series of morphological and behavioral traits to adapt to their perpetually dark and food-sparse environments. Among these traits, foraging behavior is one of the useful windows into functional advantages of behavioral trait evolution. Presented herein are updated methods for analyzing vibration attraction behavior (VAB an adaptive foraging behavior) and imaging of associated mechanosensors of cave-adapted tetra, Astyanax mexicanus. In addition, methods are presented for high-throughput tracking of a series of additional cavefish behaviors including hyperactivity and sleep-loss. Cavefish also show asociality, repetitive behavior and higher anxiety. Therefore, cavefish serve as an animal model for evolved behaviors. These methods use free-software and custom-made scripts that can be applied to other types of behavior. These methods provide practical and cost-effective alternatives to commercially available tracking software.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Comportamento Animal
/
Imageamento Tridimensional
/
Characidae
/
Cavernas
/
Neurônios
Limite:
Animals
País como assunto:
Mexico
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article