A redox sensitivity-based method to quantify both pentameric and monomeric C-reactive protein in a single assay.
J Immunol Methods
; 470: 40-45, 2019 07.
Article
em En
| MEDLINE
| ID: mdl-31034879
ABSTRACT
C-reactive protein (CRP) can exist in both pentameric (pCRP) and monomeric conformation (mCRP). Though serum pCRP is an established marker of inflammation, the diagnostic significance of mCRP remains unknown largely due to the lack of a reliable assay. The power and specificity of antibody-based assays are limited by the antibody reagents used and by the degree of cross-reactivity that may exist in detecting each antigen, as mCRP is known to be formed from the pentameric and both conformations usually coexist in clinical samples. Here, we describe an assay that measures both CRP conformations in simple samples in a single assay. This assay depends on the rationale that the intra-molecular disulfide bonds in pCRP resist reduction, while those in mCRP can be readily reduced. The distinct sensitivity of pCRP and mCRP to reduction can be easily detected and separated by electrophoresis. This assay may provide a means to study clinical correlation between pCRP and mCRP in clinical samples in the future and to evaluate their respective significance as disease markers.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Proteína C-Reativa
/
Immunoblotting
/
Eletroforese em Gel de Poliacrilamida
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article