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Physical Principles of Membrane Shape Regulation by the Glycocalyx.
Shurer, Carolyn R; Kuo, Joe Chin-Hun; Roberts, LaDeidra Monét; Gandhi, Jay G; Colville, Marshall J; Enoki, Thais A; Pan, Hao; Su, Jin; Noble, Jade M; Hollander, Michael J; O'Donnell, John P; Yin, Rose; Pedram, Kayvon; Möckl, Leonhard; Kourkoutis, Lena F; Moerner, W E; Bertozzi, Carolyn R; Feigenson, Gerald W; Reesink, Heidi L; Paszek, Matthew J.
Afiliação
  • Shurer CR; Robert Frederick Smith School of Chemical and Biomolecular Engineering, Cornell University, Ithaca, NY 14853, USA.
  • Kuo JC; Robert Frederick Smith School of Chemical and Biomolecular Engineering, Cornell University, Ithaca, NY 14853, USA.
  • Roberts LM; Meinig School of Biomedical Engineering, Cornell University, Ithaca, NY 14853, USA.
  • Gandhi JG; Robert Frederick Smith School of Chemical and Biomolecular Engineering, Cornell University, Ithaca, NY 14853, USA.
  • Colville MJ; Field of Biophysics, Cornell University, Ithaca, NY 14853, USA.
  • Enoki TA; Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA.
  • Pan H; Field of Biophysics, Cornell University, Ithaca, NY 14853, USA.
  • Su J; Department of Clinical Sciences, Cornell University, Ithaca, NY 14853, USA.
  • Noble JM; Robert Frederick Smith School of Chemical and Biomolecular Engineering, Cornell University, Ithaca, NY 14853, USA.
  • Hollander MJ; Robert Frederick Smith School of Chemical and Biomolecular Engineering, Cornell University, Ithaca, NY 14853, USA.
  • O'Donnell JP; Department of Molecular Medicine, Cornell University, Ithaca, NY 14853, USA.
  • Yin R; Robert Frederick Smith School of Chemical and Biomolecular Engineering, Cornell University, Ithaca, NY 14853, USA.
  • Pedram K; Department of Chemistry, Stanford University, Stanford, CA 94305, USA.
  • Möckl L; Department of Chemistry, Stanford University, Stanford, CA 94305, USA.
  • Kourkoutis LF; School of Applied and Engineering Physics, Cornell University, Ithaca, NY 14853, USA; Kavli Institute at Cornell for Nanoscale Science, Ithaca, NY 14853, USA.
  • Moerner WE; Department of Chemistry, Stanford University, Stanford, CA 94305, USA.
  • Bertozzi CR; Department of Chemistry, Stanford University, Stanford, CA 94305, USA; Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA.
  • Feigenson GW; Field of Biophysics, Cornell University, Ithaca, NY 14853, USA; Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA.
  • Reesink HL; Department of Clinical Sciences, Cornell University, Ithaca, NY 14853, USA.
  • Paszek MJ; Robert Frederick Smith School of Chemical and Biomolecular Engineering, Cornell University, Ithaca, NY 14853, USA; Meinig School of Biomedical Engineering, Cornell University, Ithaca, NY 14853, USA; Field of Biophysics, Cornell University, Ithaca, NY 14853, USA; Kavli Institute at Cornell for Nanosc
Cell ; 177(7): 1757-1770.e21, 2019 06 13.
Article em En | MEDLINE | ID: mdl-31056282
ABSTRACT
Cells bend their plasma membranes into highly curved forms to interact with the local environment, but how shape generation is regulated is not fully resolved. Here, we report a synergy between shape-generating processes in the cell interior and the external organization and composition of the cell-surface glycocalyx. Mucin biopolymers and long-chain polysaccharides within the glycocalyx can generate entropic forces that favor or disfavor the projection of spherical and finger-like extensions from the cell surface. A polymer brush model of the glycocalyx successfully predicts the effects of polymer size and cell-surface density on membrane morphologies. Specific glycocalyx compositions can also induce plasma membrane instabilities to generate more exotic undulating and pearled membrane structures and drive secretion of extracellular vesicles. Together, our results suggest a fundamental role for the glycocalyx in regulating curved membrane features that serve in communication between cells and with the extracellular matrix.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Glicoproteínas de Membrana / Glicocálix / Forma Celular / Matriz Extracelular / Mucinas Tipo de estudo: Prognostic_studies Limite: Animals / Humans Idioma: En Ano de publicação: 2019 Tipo de documento: Article
Texto completo
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PubMed Links
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Glicoproteínas de Membrana / Glicocálix / Forma Celular / Matriz Extracelular / Mucinas Tipo de estudo: Prognostic_studies Limite: Animals / Humans Idioma: En Ano de publicação: 2019 Tipo de documento: Article