Shape memory of in-body tissue-engineered Biotube® vascular grafts and the preliminary evaluation in animal implantation experiments.
J Cardiovasc Surg (Torino)
; 61(2): 208-213, 2020 Apr.
Article
em En
| MEDLINE
| ID: mdl-31058478
ABSTRACT
BACKGROUND:
Tissues formed by in-body tissue architecture (iBTA) are soft and flexible. However, strongly bending iBTA-induced vascular grafts, called biotubes, may cause lumen collapse by kinking, subsequently leading to occlusion after implantation. In this study, we developed a method for biotube shape memory and verified its performance in preliminary animal implantation experiments.METHODS:
Straight biotubes were prepared by subcutaneous embedding of straight molds into beagle dogs for two months. Upon overnight immersion of the obtained straight biotubes in a 70% ethanol solution under U-shape framing, the biotubes maintained their U shape even after washing with saline solution. Additionally, spiral-shaped biotubes formed from goats using spiral molds could be stretched straight via the same alcohol treatment.RESULTS:
Within limited acute-phase animal implantation experiments, U-shaped biotubes functioned as AV shunt grafts in the femoral region of the beagle without deformation of vascular shape (N.=11). In addition, the long straight biotubes derived from spiral molds could be interposed between goat carotid arteries while maintaining their straight shape (N.=2). All implants maintained perfect patency at the 1-month follow-up period without any evidence of vascular deformation.CONCLUSIONS:
By retaining iBTA-induced tissues in an alcohol solution in the target shape, the shape of the tissues was imprinted and maintained even after implantation within a limited acute period. Therefore, in order to obtain tissues of various shapes, it is unnecessary to use a mold design to maintain the individual shape.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Desenho de Prótese
/
Prótese Vascular
/
Engenharia Tecidual
/
Enxerto Vascular
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article