Bench-to-bedside optimization of mesenchymal stem cell isolation, processing, and expansion for in vivo administration.
Regen Med
; 14(4): 279-293, 2019 05.
Article
em En
| MEDLINE
| ID: mdl-31070521
ABSTRACT
Aim:
In this study, we aimed at identifying the optimal conditions for isolation, processing and expansion of mesenchymal stem cells (MSCs).Methods:
Porcine bone marrow was obtained from either small- or large-volume bone marrow aspirate (BMA). Next, three BMA processing methods were compared. Finally, the best condition was selected from various culture parameters, including basal media, supplementation and seeding density.Results:
Our results demonstrate that a small-volume BMA and direct plating yields significantly higher concentration of MSCs. Basal media supplementation with 10% platelet lysate and seeding density of 1000 cells/cm2 can generate large numbers of multipotent MSCs with augmented function and low population doublings.Conclusion:
This work provides guidance for preparation of robust MSCs for future clinical trials.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Separação Celular
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Transplante de Células-Tronco Mesenquimais
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Pesquisa Translacional Biomédica
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Células-Tronco Mesenquimais
Tipo de estudo:
Guideline
Limite:
Animals
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Female
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Humans
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article