Your browser doesn't support javascript.
loading
FAT1 cadherin controls neuritogenesis during NTera2 cell differentiation.
Ahmed, Abdulrzag F; de Bock, Charles E; Sontag, Estelle; Hondermarck, Hubert; Lincz, Lisa F; Thorne, Rick F.
Afiliação
  • Ahmed AF; Department of Pharmacology, Faculty of Pharmacy, Elmergib University, Alkhoms, Libya; School of Biomedical Sciences and Pharmacy, University of Newcastle, Callaghan, NSW, 2308, Australia.
  • de Bock CE; Children's Cancer Institute, Lowy Cancer Research Centre, UNSW Australia, Randwick, NSW 2031, Australia.
  • Sontag E; School of Biomedical Sciences and Pharmacy, University of Newcastle, Callaghan, NSW, 2308, Australia; Hunter Medical Research Institute, New Lambton, New South Wales, 2305, Australia.
  • Hondermarck H; School of Biomedical Sciences and Pharmacy, University of Newcastle, Callaghan, NSW, 2308, Australia; Hunter Medical Research Institute, New Lambton, New South Wales, 2305, Australia.
  • Lincz LF; School of Biomedical Sciences and Pharmacy, University of Newcastle, Callaghan, NSW, 2308, Australia; Hunter Medical Research Institute, New Lambton, New South Wales, 2305, Australia; Hunter Haematology Research Group, Calvary Mater Newcastle Hospital, Waratah, NSW, 2298, Australia.
  • Thorne RF; Translational Research Institute, Henan Provincial People's Hospital, Zhengzhou University, 450053, Zhengzhou, China; School of Environmental and Life Sciences, University of Newcastle, NSW, 2258, Australia. Electronic address: Rick.Thorne@newcastle.edu.au.
Biochem Biophys Res Commun ; 514(3): 625-631, 2019 06 30.
Article em En | MEDLINE | ID: mdl-31076104
ABSTRACT
Fat1 cadherin is broadly expressed throughout the nervous system and has been implicated in neuronal differentiation. Here we examined the functional contribution of FAT1 during neuronal differentiation of the Ntera2 cell line model. FAT1 expression was increased during the retinoic acid (RA)-induced differentiation of NTera2 cells. Depletion of FAT1 with siRNA decreased the number of neurites produced after RA treatment. Moreover, FAT1 silencing also led to decreased Ser127-phosphorylation of YAP along with transcriptional increases in the Hippo target genes CTGF and ANKRD1, suggesting FAT1 alters Hippo signalling during differentiation. In the context of the Ntera2 model, FAT1 is required for efficient neuritogenesis, acting as a regulator of neurite formation during the early stages of differentiation.
Assuntos
Palavras-chave

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Caderinas / Diferenciação Celular / Neuritos / Neurogênese Limite: Animals / Humans Idioma: En Ano de publicação: 2019 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Caderinas / Diferenciação Celular / Neuritos / Neurogênese Limite: Animals / Humans Idioma: En Ano de publicação: 2019 Tipo de documento: Article