Software tools for automated transmission electron microscopy.
Nat Methods
; 16(6): 471-477, 2019 06.
Article
em En
| MEDLINE
| ID: mdl-31086343
ABSTRACT
The demand for high-throughput data collection in electron microscopy is increasing for applications in structural and cellular biology. Here we present a combination of software tools that enable automated acquisition guided by image analysis for a variety of transmission electron microscopy acquisition schemes. SerialEM controls microscopes and detectors and can trigger automated tasks at multiple positions with high flexibility. Py-EM interfaces with SerialEM to enact specimen-specific image-analysis pipelines that enable feedback microscopy. As example applications, we demonstrate dose reduction in cryo-electron microscopy experiments, fully automated acquisition of every cell in a plastic section and automated targeting on serial sections for 3D volume imaging across multiple grids.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Processamento de Imagem Assistida por Computador
/
Software
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Microscopia Eletrônica de Transmissão
Limite:
Humans
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article