Hydrolyzable Tannins Are Iron Chelators That Inhibit DNA Repair Enzyme ALKBH2.
Chem Res Toxicol
; 32(6): 1082-1086, 2019 06 17.
Article
em En
| MEDLINE
| ID: mdl-31088072
ABSTRACT
Hydrolyzable tannins are a class of polyphenolic compounds commonly found in natural products. In this work, we studied the in vitro inhibitory mechanism of six molecules in this class on ALKBH2, an Fe(II)/α-ketoglutarate-dependent DNA repair enzyme in the AlkB family. We determined the IC50 values of these compounds on the repair of 3-methylcytosine and 1-methyladenine, the prototypical substrates of ALKBH2. A structure-activity relationship was also observed between the strength of inhibition and the number of galloyl moieties in a molecule. In addition, we found that the inhibition by this class of polyphenolic compounds on ALKBH2 is through an iron-chelating mechanism.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Quelantes de Ferro
/
Taninos Hidrolisáveis
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Reparo do DNA
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Inibidores Enzimáticos
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Homólogo AlkB 2 da Dioxigenase Dependente de alfa-Cetoglutarato
Limite:
Humans
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article