Prevalence of colistin resistance and mcr-1/mcr-2 genes in extended-spectrum ß-lactamase/AmpC-producing Escherichia coli isolated from chickens in Canada, Senegal and Vietnam.
J Glob Antimicrob Resist
; 19: 222-227, 2019 12.
Article
em En
| MEDLINE
| ID: mdl-31100501
ABSTRACT
OBJECTIVES:
This study investigated the prevalence of Escherichia coli (E. coli) colistin resistance and mcr-1 and mcr-2 genes among extended-spectrum ß-lactamase (ESBL)/AmpC-producing E. coli isolates recovered from chicken feces in Canada (Quebec), Senegal and Vietnam, and evaluated the susceptibility pattern of the colistin-resistant E. coli isolates to other clinically relevant antimicrobials.METHODS:
A total of 327 potential ESBL/AmpC-producing E. coli isolates from chicken farms in Canada (Quebec), Senegal and Vietnam were analysed for colistin susceptibility by broth microdilution method and for the presence of mcr (1-2) genes by PCR. The pmrA and pmrB genes of colistin-resistant E. coli isolates, in the absence of mcr (1-2) genes, were sequenced. Antimicrobial resistance phenotypes of colistin-resistant E. coli isolates were determined by disk diffusion.RESULTS:
None of the 108 potential ESBL/AmpC-producing E. coli isolates from seven farms in Canada were colistin-resistant or possessed mcr-1 or mcr-2 gene. A low prevalence of 2.2% of colistin resistance was observed in 93 Senegalese isolates from the 15 sampled farms, although neither mcr-1 nor mcr-2 gene was found. A prevalence of 8.7% of colistin resistance was observed among 126 Vietnamese isolates from two of the four sampled farms. The mcr-1 gene was detected in 85% of the 13 phenotypically colistin-resistant isolates. Moreover, all colistin-resistant isolates presented a multidrug-resistant phenotype.CONCLUSIONS:
The co-existence of the mcr-1 and ESBL/AmpC genes and the very high level of multiple drug resistance in all colistin-resistant E. coli isolates obtained from sampled chicken farms in Vietnam is a major concern.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Doenças das Aves Domésticas
/
Proteínas de Bactérias
/
Beta-Lactamases
/
Colistina
/
Proteínas de Escherichia coli
/
Escherichia coli
/
Infecções por Escherichia coli
Tipo de estudo:
Prevalence_studies
/
Risk_factors_studies
Limite:
Animals
País como assunto:
Africa
/
America do norte
/
Asia
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article