Conformational Complexity and Dynamics in a Muscarinic Receptor Revealed by NMR Spectroscopy.

Xu, Jun; Hu, Yunfei; Kaindl, Jonas; Risel, Philipp; Hübner, Harald; Maeda, Shoji; Niu, Xiaogang; Li, Hongwei; Gmeiner, Peter; Jin, Changwen; Kobilka, Brian K

Xu, Jun; Hu, Yunfei; Kaindl, Jonas; Risel, Philipp; Hübner, Harald; Maeda, Shoji; Niu, Xiaogang; Li, Hongwei; Gmeiner, Peter; Jin, Changwen; Kobilka, Brian K.

Afiliação

Xu J; Beijing Advanced Innovation Center for Structural Biology, School of Medicine, Tsinghua University, Beijing 100084, China.
Hu Y; Beijing Nuclear Magnetic Resonance Center, College of Chemistry and Molecular Engineering, School of Life Sciences, Peking University, Beijing 100084, China.
Kaindl J; Department of Chemistry and Pharmacy, Medicinal Chemistry, Friedrich-Alexander-Universität Erlangen-Nürnberg, Nikolaus-Fiebiger-Straße 10, 91058 Erlangen, Germany.
Risel P; Department of Chemistry and Pharmacy, Medicinal Chemistry, Friedrich-Alexander-Universität Erlangen-Nürnberg, Nikolaus-Fiebiger-Straße 10, 91058 Erlangen, Germany.
Hübner H; Department of Chemistry and Pharmacy, Medicinal Chemistry, Friedrich-Alexander-Universität Erlangen-Nürnberg, Nikolaus-Fiebiger-Straße 10, 91058 Erlangen, Germany.
Maeda S; Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, CA 94305, USA.
Niu X; Beijing Nuclear Magnetic Resonance Center, College of Chemistry and Molecular Engineering, School of Life Sciences, Peking University, Beijing 100084, China.
Li H; Beijing Nuclear Magnetic Resonance Center, College of Chemistry and Molecular Engineering, School of Life Sciences, Peking University, Beijing 100084, China.
Gmeiner P; Department of Chemistry and Pharmacy, Medicinal Chemistry, Friedrich-Alexander-Universität Erlangen-Nürnberg, Nikolaus-Fiebiger-Straße 10, 91058 Erlangen, Germany.
Jin C; Beijing Nuclear Magnetic Resonance Center, College of Chemistry and Molecular Engineering, School of Life Sciences, Peking University, Beijing 100084, China. Electronic address: changwen@pku.edu.cn.
Kobilka BK; Beijing Advanced Innovation Center for Structural Biology, School of Medicine, Tsinghua University, Beijing 100084, China; Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, CA 94305, USA. Electronic address: kobilka@stanford.edu.

Mol Cell ; 75(1): 53-65.e7, 2019 07 11.

Article em En | MEDLINE | ID: mdl-31103421

Assuntos

Acetilcolina/química; Carbacol/química; Isoxazóis/química; Pilocarpina/química; Piridinas/química; Compostos de Amônio Quaternário/química; Receptor Muscarínico M2/química; Tiadiazóis/química; Acetilcolina/metabolismo; Animais; Baculoviridae/genética; Baculoviridae/metabolismo; Sítios de Ligação; Carbacol/metabolismo; Clonagem Molecular; Expressão Gênica; Vetores Genéticos/química; Vetores Genéticos/metabolismo; Humanos; Isoxazóis/metabolismo; Cinética; Ligantes; Espectroscopia de Ressonância Magnética; Simulação de Dinâmica Molecular; Pilocarpina/metabolismo; Ligação Proteica; Conformação Proteica em alfa-Hélice; Conformação Proteica em Folha beta; Domínios e Motivos de Interação entre Proteínas; Piridinas/metabolismo; Compostos de Amônio Quaternário/metabolismo; Receptor Muscarínico M2/agonistas; Receptor Muscarínico M2/genética; Receptor Muscarínico M2/metabolismo; Proteínas Recombinantes/química; Proteínas Recombinantes/genética; Proteínas Recombinantes/metabolismo; Células Sf9; Spodoptera; Termodinâmica; Tiadiazóis/metabolismo

Palavras-chave

GPCR signal transduction; NMR spectroscopy; ligand efficacy; signaling bias; structure and dynamics of a muscarinic receptor

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Pilocarpina / Piridinas / Tiadiazóis / Carbacol / Acetilcolina / Receptor Muscarínico M2 / Compostos de Amônio Quaternário / Isoxazóis Idioma: En Ano de publicação: 2019 Tipo de documento: Article

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