Induced pluripotent stem cell line heterozygous for p.R2447X mutation in filaggrin: KCLi002-A.
Stem Cell Res
; 38: 101462, 2019 07.
Article
em En
| MEDLINE
| ID: mdl-31103941
ABSTRACT
We have generated an induced pluripotent stem cell (iPSC) line KCLi002-A (iOP107) from a female donor, heterozygous for the loss-of-function mutation p.R2447X in the filaggrin gene (FLG). Epidermal keratinocytes were reprogrammed using non-integrating Sendai virus vectors. The entire process of derivation and expansion of iPSCs were performed under xeno-free culture conditions. Characterization of KCLi002-A line included molecular karyotyping, mutation screening using restriction enzyme digestion Sanger sequencing and next generation sequencing (NGS), whereas pluripotency and differentiation potential were confirmed by expression of associated markers in vitro and in vivo teratoma assay.
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Base de dados:
MEDLINE
Assunto principal:
Proteínas S100
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Mutação de Sentido Incorreto
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Células-Tronco Pluripotentes Induzidas
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Mutação com Perda de Função
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Heterozigoto
Limite:
Female
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Humans
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article