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Selection of Biophysical Methods for Characterisation of Membrane Proteins.
Kwan, Tristan O C; Reis, Rosana; Siligardi, Giuliano; Hussain, Rohanah; Cheruvara, Harish; Moraes, Isabel.
Afiliação
  • Kwan TOC; National Physical Laboratory, Hampton Road, Teddington TW11 0LW, UK. tristan.kwan@npl.co.uk.
  • Reis R; Research Complex at Harwell, Rutherford Appleton Laboratory, Harwell Science and Innovation Campus, Didcot OX11 0FA, UK. tristan.kwan@npl.co.uk.
  • Siligardi G; National Physical Laboratory, Hampton Road, Teddington TW11 0LW, UK. rosana.thevenot@npl.co.uk.
  • Hussain R; Research Complex at Harwell, Rutherford Appleton Laboratory, Harwell Science and Innovation Campus, Didcot OX11 0FA, UK. rosana.thevenot@npl.co.uk.
  • Cheruvara H; Diamond Light Source Ltd., Harwell Science and Innovation Campus, Didcot OX11 0DE, UK. giuliano.siligardi@diamond.ac.uk.
  • Moraes I; Diamond Light Source Ltd., Harwell Science and Innovation Campus, Didcot OX11 0DE, UK. rohanah.hussain@diamond.ac.uk.
Int J Mol Sci ; 20(10)2019 May 27.
Article em En | MEDLINE | ID: mdl-31137900
ABSTRACT
Over the years, there have been many developments and advances in the field of integral membrane protein research. As important pharmaceutical targets, it is paramount to understand the mechanisms of action that govern their structure-function relationships. However, the study of integral membrane proteins is still incredibly challenging, mostly due to their low expression and instability once extracted from the native biological membrane. Nevertheless, milligrams of pure, stable, and functional protein are always required for biochemical and structural studies. Many modern biophysical tools are available today that provide critical information regarding to the characterisation and behaviour of integral membrane proteins in solution. These biophysical approaches play an important role in both basic research and in early-stage drug discovery processes. In this review, it is not our objective to present a comprehensive list of all existing biophysical methods, but a selection of the most useful and easily applied to basic integral membrane protein research.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Dicroísmo Circular / Recuperação de Fluorescência Após Fotodegradação / Difusão Dinâmica da Luz / Proteínas de Membrana Limite: Animals / Humans Idioma: En Ano de publicação: 2019 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Dicroísmo Circular / Recuperação de Fluorescência Após Fotodegradação / Difusão Dinâmica da Luz / Proteínas de Membrana Limite: Animals / Humans Idioma: En Ano de publicação: 2019 Tipo de documento: Article