A new protocol for psychrometric pressure-volume curves of fern gametophytes.
Appl Plant Sci
; 7(5): e01248, 2019 May.
Article
em En
| MEDLINE
| ID: mdl-31139514
ABSTRACT
PREMISE Pressure-volume curves are a widely used analytical framework to derive several key physiological traits related to plant-water relations, including a species' turgor loss point, osmotic potential at full turgor, and the elasticity of cell walls. We developed a novel protocol, including the preparation and treatment of fern gametophytes, to generate data for pressure-volume curve analyses using thermocouple psychrometry. METHODS AND RESULTS:
Gametophytes of the fern species Polystichum lemmonii were grown from spore, harvested, and subjected to a series of drying intervals. We constructed pressure-volume curves using thermocouple psychrometers to calculate gametophyte water potential and a balance to measure relative water loss.CONCLUSIONS:
We present the first protocol for fern gametophyte pressure-volume curves that can accurately determine key physiological traits in fern gametophytes such as the turgor loss point and osmotic potential at full turgor.
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MEDLINE
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Guideline
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article