Cation exchange chromatography performed in overloaded mode is effective in removing viruses during the manufacturing of monoclonal antibodies.

Masuda, Yumiko; Tsuda, Masashi; Hashikawa-Muto, Chie; Takahashi, Yusuke; Nonaka, Koichi; Wakamatsu, Kaori

Masuda, Yumiko; Tsuda, Masashi; Hashikawa-Muto, Chie; Takahashi, Yusuke; Nonaka, Koichi; Wakamatsu, Kaori.

Afiliação

Masuda Y; Biologics Technology Research Laboratories, Daiichi Sankyo Co., Ltd., Ohra-gun, Gunma, Japan.
Tsuda M; Graduate School of Science and Technology, Gunma University, Kiryu-shi, Gunma, Japan.
Hashikawa-Muto C; Biologics Technology Research Laboratories, Daiichi Sankyo Co., Ltd., Ohra-gun, Gunma, Japan.
Takahashi Y; Biologics Technology Research Laboratories, Daiichi Sankyo Co., Ltd., Ohra-gun, Gunma, Japan.
Nonaka K; Biologics Technology Research Laboratories, Daiichi Sankyo Co., Ltd., Ohra-gun, Gunma, Japan.
Wakamatsu K; Biologics Technology Research Laboratories, Daiichi Sankyo Co., Ltd., Ohra-gun, Gunma, Japan.

Biotechnol Prog ; 35(5): e2858, 2019 09.

Article em En | MEDLINE | ID: mdl-31148380

Assuntos

Anticorpos Monoclonais; Cromatografia por Troca Iônica/métodos; Vírus/isolamento & purificação; Animais; Anticorpos Monoclonais/análise; Anticorpos Monoclonais/metabolismo; Biotecnologia/métodos; Células CHO; Cátions/química; Cricetinae; Cricetulus

Palavras-chave

cation exchange chromatography; monoclonal antibody; overloaded mode; purification; viral clearance

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Vírus / Cromatografia por Troca Iônica / Anticorpos Monoclonais Limite: Animals Idioma: En Ano de publicação: 2019 Tipo de documento: Article

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