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Photolysis of Staphyloxanthin in Methicillin-Resistant Staphylococcus aureus Potentiates Killing by Reactive Oxygen Species.
Dong, Pu-Ting; Mohammad, Haroon; Hui, Jie; Leanse, Leon G; Li, Junjie; Liang, Lijia; Dai, Tianhong; Seleem, Mohamed N; Cheng, Ji-Xin.
Afiliação
  • Dong PT; Department of Chemistry Boston University Boston MA 02215 USA.
  • Mohammad H; Department of Comparative Pathobiology College of Veterinary Medicine Purdue University West Lafayette IN 47907 USA.
  • Hui J; Prof. J.-X. Cheng, Department of Electrical and Computer Engineering Boston University Boston MA 02215 USA.
  • Leanse LG; Wellman Center for Photomedicine Massachusetts General Hospital Harvard Medical School MA 02114 USA.
  • Li J; Prof. J.-X. Cheng, Department of Electrical and Computer Engineering Boston University Boston MA 02215 USA.
  • Liang L; State Key Laboratory of Supramolecular Structure and Materials Institute of Theoretical Chemistry Jilin University Changchun 130012 China.
  • Dai T; Wellman Center for Photomedicine Massachusetts General Hospital Harvard Medical School MA 02114 USA.
  • Seleem MN; Department of Comparative Pathobiology College of Veterinary Medicine Purdue University West Lafayette IN 47907 USA.
  • Cheng JX; Department of Chemistry Boston University Boston MA 02215 USA.
Adv Sci (Weinh) ; 6(11): 1900030, 2019 Jun 05.
Article em En | MEDLINE | ID: mdl-31179216
ABSTRACT
Confronted with the severe situation that the pace of resistance acquisition is faster than the clinical introduction of new antibiotics, health organizations are calling for effective approaches to combat methicillin-resistant Staphylococcus aureus (MRSA) infections. Here, an approach to treat MRSA through photolysis of staphyloxanthin, an antioxidant residing in the microdomain of S. aureus membrane, is reported. This photochemistry process is uncovered through transient absorption imaging and quantitated by absorption spectroscopy, Raman spectroscopy, and mass spectrometry. Photolysis of staphyloxanthin transiently elevates the membrane permeability and renders MRSA highly susceptible to hydrogen peroxide attack. Consequently, staphyloxanthin photolysis by low-level 460 nm light eradicates MRSA synergistically with hydrogen peroxide and other reactive oxygen species. The effectiveness of this synergistic therapy is well validated in MRSA planktonic culture, MRSA-infected macrophage cells, stationary-phase MRSA, persisters, S. aureus biofilms, and two mice wound infection models. Collectively, the work demonstrates that staphyloxanthin photolysis is a new therapeutic platform to treat MRSA infections.
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Texto completo: 1 Base de dados: MEDLINE Idioma: En Ano de publicação: 2019 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Idioma: En Ano de publicação: 2019 Tipo de documento: Article