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A Simple HPLC Method for the Quantitative Determination of Silybin in Rat Plasma: Application to a Comparative Pharmacokinetic Study on Commercial Silymarin Products.
Ha, Eun-Sol; Han, Dong-Gyun; Seo, Seong-Wook; Kim, Ji-Min; Lee, Seon-Kwang; Sim, Woo-Yong; Yoon, In-Soo; Kim, Min-Soo.
Afiliação
  • Ha ES; Department of Manufacturing Pharmacy, College of Pharmacy, Pusan National University, Busan 46241, Korea. edel@pusan.ac.kr.
  • Han DG; Department of Manufacturing Pharmacy, College of Pharmacy, Pusan National University, Busan 46241, Korea. hann9607@pusan.ac.kr.
  • Seo SW; Department of Manufacturing Pharmacy, College of Pharmacy, Pusan National University, Busan 46241, Korea. sswook@pusan.ac.kr.
  • Kim JM; Department of Manufacturing Pharmacy, College of Pharmacy, Pusan National University, Busan 46241, Korea. jiminkim@pusan.ac.kr.
  • Lee SK; Department of Manufacturing Pharmacy, College of Pharmacy, Pusan National University, Busan 46241, Korea. lsk7079@pusan.ac.kr.
  • Sim WY; Department of Manufacturing Pharmacy, College of Pharmacy, Pusan National University, Busan 46241, Korea. popo923@pusan.ac.kr.
  • Yoon IS; Department of Manufacturing Pharmacy, College of Pharmacy, Pusan National University, Busan 46241, Korea. insoo.yoon@pusan.ac.kr.
  • Kim MS; Department of Manufacturing Pharmacy, College of Pharmacy, Pusan National University, Busan 46241, Korea. minsookim@pusan.ac.kr.
Molecules ; 24(11)2019 Jun 10.
Article em En | MEDLINE | ID: mdl-31185663
Silybin (SBN) is a major active constituent of silymarin, a mixture of flavonoids found in fruits and seeds of milk thistle. The aim of this study was to describe a simple bioanalytical method for quantifying SBN in rat plasma. A simple protein deproteinization procedure with acetonitrile (ACN) was employed for plasma sample preparation. A reversed column and gradient elution of a mobile phase (mixture of phosphate buffer (pH 5.0) and ACN) were used for chromatographic separation. The selectivity, linearity (50-5000 ng/mL), precision, accuracy, recovery, matrix effect, and stability for this method were validated as per the current Food and Drug Administration (FDA) guidelines. Our method for SBN was applied to a comparative pharmacokinetic study on four different commercial silymarin products. This in vivo rat study demonstrated that product #4 significantly enhanced the relative oral bioavailability of SBN, as compared to product #1-3. Therefore, the bioanalytical method proposed herein could serve as a promising alternative for preclinical pharmacokinetic studies on silymarin products and, by extension, clinical use after partial modification and validation.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Cromatografia Líquida de Alta Pressão / Silibina Tipo de estudo: Guideline Limite: Animals Idioma: En Ano de publicação: 2019 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Cromatografia Líquida de Alta Pressão / Silibina Tipo de estudo: Guideline Limite: Animals Idioma: En Ano de publicação: 2019 Tipo de documento: Article