An acyl-CoA dehydrogenase microplate activity assay using recombinant porcine electron transfer flavoprotein.

Zhang, Yuxun; Mohsen, Al-Walid; Kochersperger, Catherine; Solo, Keaton; Schmidt, Alexandra V; Vockley, Jerry; Goetzman, Eric S

Zhang, Yuxun; Mohsen, Al-Walid; Kochersperger, Catherine; Solo, Keaton; Schmidt, Alexandra V; Vockley, Jerry; Goetzman, Eric S.

Afiliação

Zhang Y; Department of Pediatrics, Division of Medical Genetics, University of Pittsburgh School of Medicine, Children's Hospital of Pittsburgh, 4401 Penn Avenue, Pittsburgh, PA, 15224, USA.
Mohsen AW; Department of Pediatrics, Division of Medical Genetics, University of Pittsburgh School of Medicine, Children's Hospital of Pittsburgh, 4401 Penn Avenue, Pittsburgh, PA, 15224, USA.
Kochersperger C; Department of Pediatrics, Division of Medical Genetics, University of Pittsburgh School of Medicine, Children's Hospital of Pittsburgh, 4401 Penn Avenue, Pittsburgh, PA, 15224, USA.
Solo K; Department of Pediatrics, Division of Medical Genetics, University of Pittsburgh School of Medicine, Children's Hospital of Pittsburgh, 4401 Penn Avenue, Pittsburgh, PA, 15224, USA.
Schmidt AV; Department of Pediatrics, Division of Medical Genetics, University of Pittsburgh School of Medicine, Children's Hospital of Pittsburgh, 4401 Penn Avenue, Pittsburgh, PA, 15224, USA.
Vockley J; Department of Pediatrics, Division of Medical Genetics, University of Pittsburgh School of Medicine, Children's Hospital of Pittsburgh, 4401 Penn Avenue, Pittsburgh, PA, 15224, USA.
Goetzman ES; Department of Pediatrics, Division of Medical Genetics, University of Pittsburgh School of Medicine, Children's Hospital of Pittsburgh, 4401 Penn Avenue, Pittsburgh, PA, 15224, USA. Electronic address: eric.goetzman@chp.edu.

Anal Biochem ; 581: 113332, 2019 09 15.

Article em En | MEDLINE | ID: mdl-31194945

Assuntos

Acil-CoA Desidrogenases/química; Flavoproteínas Transferidoras de Elétrons/química; Acil-CoA Desidrogenases/genética; Animais; Flavoproteínas Transferidoras de Elétrons/genética; Escherichia coli/química; Escherichia coli/genética; Ácidos Graxos/química; Ácidos Graxos/metabolismo; Proteínas Recombinantes/química; Proteínas Recombinantes/genética; Suínos

Palavras-chave

Acyl-CoA dehydrogenase; Electron transfer flavoprotein; Enzyme activity assay; Fatty acid oxidation; Mitochondria

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Flavoproteínas Transferidoras de Elétrons / Acil-CoA Desidrogenases Tipo de estudo: Guideline Limite: Animals Idioma: En Ano de publicação: 2019 Tipo de documento: Article

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