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Combined delivery of VEGF and IGF-1 promotes functional innervation in mice and improves muscle transplantation in rabbits.
Raimondo, Theresa M; Li, Hehuan; Kwee, Brian J; Kinsley, Sarah; Budina, Erica; Anderson, Erin M; Doherty, Edward J; Talbot, Simon G; Mooney, David J.
Afiliação
  • Raimondo TM; John A. Paulson School of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138, USA; Wyss Institute for Biologically Inspired Engineering at Harvard University, Cambridge,MA 02138, USA.
  • Li H; Division of Plastic Surgery, Department of Surgery, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA; Department of Plastic Surgery, The First Hospital of China Medical University, Shenyang, China.
  • Kwee BJ; John A. Paulson School of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138, USA; Wyss Institute for Biologically Inspired Engineering at Harvard University, Cambridge,MA 02138, USA.
  • Kinsley S; Division of Plastic Surgery, Department of Surgery, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.
  • Budina E; John A. Paulson School of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138, USA.
  • Anderson EM; John A. Paulson School of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138, USA; Wyss Institute for Biologically Inspired Engineering at Harvard University, Cambridge,MA 02138, USA.
  • Doherty EJ; Wyss Institute for Biologically Inspired Engineering at Harvard University, Cambridge,MA 02138, USA.
  • Talbot SG; Division of Plastic Surgery, Department of Surgery, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA. Electronic address: SGTALBOT@bwh.harvard.edu.
  • Mooney DJ; John A. Paulson School of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138, USA; Wyss Institute for Biologically Inspired Engineering at Harvard University, Cambridge,MA 02138, USA. Electronic address: mooneyd@seas.harvard.edu.
Biomaterials ; 216: 119246, 2019 09.
Article em En | MEDLINE | ID: mdl-31203034
Microvascular muscle transfer is the gold standard for reanimation following chronic facial nerve paralysis, however, despite the regenerative capacity of peripheral motor axons, poor reinnervation often results in sub-optimal function. We hypothesized that injection of alginate hydrogels releasing growth factors directly into donor tissue would promote reinnervation, muscle regeneration, and function. A murine model of sciatic nerve ligation and neurorrhaphy was first used to assess the ability of gel delivery of vascular endothelial growth factor (VEGF) and insulin-like growth factor-1 (IGF-1) to promote functional reinnervation. VEGF + IGF-1 gel delivery to aged mice resulted in prolonged ability to control toe movement, increased toe spreading, and improved static sciatic index score, indicative of improved sciatic nerve and neuromuscular junction function. Further, a 26% increase in muscle fiber area, and 2.8 and 3.0-fold increases in muscle contraction force and velocity, respectively, were found compared to blank alginate in the murine model. This strategy was subsequently tested in a rabbit model of craniofacial gracilis muscle transplantation. Electromyography demonstrated a 71% increase in compound muscle action potential 9 weeks after transplantation following treatment with VEGF + IGF-1 alginate, compared to blank alginate in the rabbit model. Improving functional innervation in transplanted muscle via a hydrogel source of growth factors may enhance the therapeutic outcomes of facial palsy treatments and, more broadly, muscle transplantations.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Fator de Crescimento Insulin-Like I / Sistemas de Liberação de Medicamentos / Músculo Esquelético / Fator A de Crescimento do Endotélio Vascular Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Ano de publicação: 2019 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Fator de Crescimento Insulin-Like I / Sistemas de Liberação de Medicamentos / Músculo Esquelético / Fator A de Crescimento do Endotélio Vascular Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Ano de publicação: 2019 Tipo de documento: Article