Biological and molecular characterization of Rachiplusia nu single nucleopolyhedrovirus, a promising biocontrol agent against the South American soybean pest Rachiplusia nu.
J Invertebr Pathol
; 166: 107211, 2019 09.
Article
em En
| MEDLINE
| ID: mdl-31220457
ABSTRACT
Rachiplusia nu (Lepidoptera Noctuidae) is a key soybean pest in Argentina. Current management of this moth relies mainly on the use of synthetic insecticides and transgenic plants. In search of biological control-based alternatives, a baculovirus from R. nu (hereafter RanuNPV) was characterized and its insecticidal properties tested under laboratory conditions. RanuNPV occlusion bodies (OBs) were nearly tetrahedral, averaging 1.0⯱â¯0.2⯵m in their longest edge and containing singly enveloped nucleocapsids. Histopathology of infected late-instar larvae revealed broad tissue tropism, where fat bodies and epidermis were the most affected organs. Phylogenetic analysis of concatenated polh, lef-8 and lef-9 partial sequences classified RanuNPV as a new species that clusters with other group II alphabaculoviruses infecting larvae of Plusiinae. Bioassays performed with R. nu neonates determined the median lethal dosage to be approximately 2.5â¯OBs/larva; most insects died within 4-5â¯days post inoculation showing typical baculovirus-induced liquefaction. No effects were observed in other lepidopteran species assayed, including Spodoptera frugiperda, Cydia pomonella and Diatraea saccharalis. High pathogenicity and host specificity make RanuNPV a good candidate for controlling R. nu.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Controle Biológico de Vetores
/
Nucleopoliedrovírus
/
Mariposas
Limite:
Animals
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article