Recording development with single cell dynamic lineage tracing.
Development
; 146(12)2019 06 27.
Article
em En
| MEDLINE
| ID: mdl-31249005
ABSTRACT
Every animal grows from a single fertilized egg into an intricate network of cell types and organ systems. This process is captured in a lineage tree a diagram of every cell's ancestry back to the founding zygote. Biologists have long sought to trace this cell lineage tree in individual organisms and have developed a variety of technologies to map the progeny of specific cells. However, there are billions to trillions of cells in complex organisms, and conventional approaches can only map a limited number of clonal populations per experiment. A new generation of tools that use molecular recording methods integrated with single cell profiling technologies may provide a solution. Here, we summarize recent breakthroughs in these technologies, outline experimental and computational challenges, and discuss biological questions that can be addressed using single cell dynamic lineage tracing.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Biologia do Desenvolvimento
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Linhagem da Célula
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Análise de Célula Única
Limite:
Animals
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Humans
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article