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Establishment of a system for screening autophagic flux regulators using a modified fluorescent reporter and CRISPR/Cas9.
Yazawa, Rieko; Nishida, Yuya; Aoyama, Shuhei; Tanida, Isei; Miyatsuka, Takeshi; Suzuki, Luka; Himuro, Miwa; Haruna, Hidenori; Takubo, Noriyuki; Shimizu, Toshiaki; Watada, Hirotaka.
Afiliação
  • Yazawa R; Department of Pediatrics and Adolescent Medicine, Juntendo University Graduate School of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo, 113-8421, Japan.
  • Nishida Y; Department of Endocrinology and Metabolism, Juntendo University Graduate School of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo, 113-8421, Japan; Center for Therapeutic Innovations in Diabetes, 2-1-1 Hongo, Bunkyo-ku, Tokyo, 113-8421, Japan. Electronic address: y-nishida@juntendo.ac.jp.
  • Aoyama S; Department of Endocrinology and Metabolism, Juntendo University Graduate School of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo, 113-8421, Japan.
  • Tanida I; Department of Cellular and Molecular Neuropathology, Juntendo University Graduate School of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo, 113-8421, Japan.
  • Miyatsuka T; Department of Endocrinology and Metabolism, Juntendo University Graduate School of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo, 113-8421, Japan; Center for Identification of Diabetic Therapeutic Targets, 2-1-1 Hongo, Bunkyo-ku, Tokyo, 113-8421, Japan.
  • Suzuki L; Department of Endocrinology and Metabolism, Juntendo University Graduate School of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo, 113-8421, Japan; Center for Identification of Diabetic Therapeutic Targets, 2-1-1 Hongo, Bunkyo-ku, Tokyo, 113-8421, Japan.
  • Himuro M; Department of Endocrinology and Metabolism, Juntendo University Graduate School of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo, 113-8421, Japan.
  • Haruna H; Department of Pediatrics and Adolescent Medicine, Juntendo University Graduate School of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo, 113-8421, Japan.
  • Takubo N; Department of Pediatrics and Adolescent Medicine, Juntendo University Graduate School of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo, 113-8421, Japan.
  • Shimizu T; Department of Pediatrics and Adolescent Medicine, Juntendo University Graduate School of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo, 113-8421, Japan.
  • Watada H; Department of Endocrinology and Metabolism, Juntendo University Graduate School of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo, 113-8421, Japan; Center for Therapeutic Innovations in Diabetes, 2-1-1 Hongo, Bunkyo-ku, Tokyo, 113-8421, Japan; Center for Identification of Diabetic Therapeutic Targets, 2-1-
Biochem Biophys Res Commun ; 516(3): 686-692, 2019 08 27.
Article em En | MEDLINE | ID: mdl-31253397
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Autofagia / Proteínas de Fluorescência Verde / Sistemas CRISPR-Cas / Proteínas Luminescentes / Proteínas Associadas aos Microtúbulos Tipo de estudo: Diagnostic_studies / Screening_studies Limite: Animals / Humans Idioma: En Ano de publicação: 2019 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Autofagia / Proteínas de Fluorescência Verde / Sistemas CRISPR-Cas / Proteínas Luminescentes / Proteínas Associadas aos Microtúbulos Tipo de estudo: Diagnostic_studies / Screening_studies Limite: Animals / Humans Idioma: En Ano de publicação: 2019 Tipo de documento: Article