Quantitative analysis of super-resolved structures using ASAP.
Nat Methods
; 16(8): 711-714, 2019 08.
Article
em En
| MEDLINE
| ID: mdl-31263253
ABSTRACT
Super-resolution microscopy allows imaging of cellular structures with high throughput and detail. However, the efficient and quantitative analysis of images generated is challenging with existing tools. Here, we develop ASAP (automated structures analysis program) to enable rapid and automated detection, classification and quantification of super-resolved structures. We validate ASAP on ground truth data and demonstrate its broad applicability by analyzing images of nucleoporins, TORC1 complexes, endocytic vesicles and Bax pores.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Endossomos
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Vesículas Transportadoras
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Complexo de Proteínas Formadoras de Poros Nucleares
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Proteína X Associada a bcl-2
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Imagem Molecular
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Alvo Mecanístico do Complexo 1 de Rapamicina
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Microscopia de Fluorescência
Limite:
Humans
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article