pUC18-CpG stimulates RAW 264.7 via TBK1-mediated pathway and presents adjuvanticity in mice.
Pol J Vet Sci
; 22(2): 195-201, 2019 Jun.
Article
em En
| MEDLINE
| ID: mdl-31269330
ABSTRACT
Phosphorothioate CpG oligodeoxynucleotides (ODN) are reported to be recognized by the membrane-bound TLR9 and trigger the MyD88-dependent up-regulation of Type I interferons and pro-inflammatory cytokines. Whether plasmids containing multiple CpG motifs stimulate the same signaling pathway is yet to be determined. The present results show that the CpG motifs enrich plasmid pUC18-CpG stimulates RAW 264.7 in vitro, mainly through the TBK1-mediated signaling pathway, causing the up-regulation of IFN-ß, and pro-inflammatory cytokines TNF-α and IL-6. When pUC18-CpG is co-administered with the recombinant Echinococcus granulosus antigen, the antigen-specific antibody titers are markedly increased compared to the Quil-A adju- vanted group. Antigen specific cytokine quantification shows that cytokine profiles from the pUC18-CpG adjuvanted-group are switched to a Th1-biased immune response.
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Base de dados:
MEDLINE
Assunto principal:
Oligodesoxirribonucleotídeos
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Anticorpos Anti-Helmínticos
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Proteínas Serina-Treonina Quinases
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Antígenos de Helmintos
Limite:
Animals
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article